What are Exosomes?

•  Exosomes are small vesicles ranging from 30-120 nm (nanometers) in size that
are found in nearly all eukaryo?c fluids and facilitate a range of important cellular
func?ons. They transfer…
•  DNA
•  RNA (mRNA and miRNA)
•  Proteins (cytochines, chemokines, etc)

•  Thereby altering the func?on of the target cells
•  For decades, exosomes were considered extracellular by products, but these
nano-sized vesicles are now becoming increasingly recognized for their cri?cal
role in intercellular communica?on for both healthy and diseased cells!

Exosomes- Messengers of the Body
•  Extracellular Vesicles (E.V.s) which consist of exosomes, macrovesicles, and apopto?c
bodies, release directly from the cell membrane into the intracellular matrix
•  EVs mediate cell-to-cell communica?on, and are involved in many processes including:

•  Immune signaling
•  Angiogenesis
•  Prolifera?on
•  Cell differen?a?on
•  Stress response

•  EVs are used in a paracrine signaling to regulate cell differen?a?on, prolifera?on, and
recruitment via the molecules that are stored within the vesicles
•  Exosomes can travel through different bodily fluids such as the blood stream to reach cells
in many different parts of the body to facilitate cell-to-cell communica?on

Cargo-Growth Factors and Cytokines
•  Growth factors are proteins that can locally and systemically affect
the growth of cell in mul?ple ways
•  Cellular division is influenced by growth factors
•  Cytokines are another protein that are responsible for regula?ng the
immune response, inflamma?on, ?ssue remolding, and cell
differen?a?on
•  Growth factors and Cytokines guide cell differen?a?on and
regenera?on in all organs and ?ssues

Examples of contents/growth factors identified in UCB-PRP
Exosomes:
•  NID1- extracellular matrix organiza5on, basement membrane organiza5on, posi5ve regula5on of cell-substrate adhesion
•  KRT10- kera5nocyte differen5a5on
•  KRT2- kera5niza5on, epidermis development, kera5nocyte prolifera5on
•  ZNF503- neural precursor cell prolifera5on
•  CDH13- cell junc5on assembly, endothelial cell migra5on, sprou5ng angiogenesis, posi5ve regula5on of: cell prolifera5on, cell-
•  CTSG- angiotension matura5on, extracellular matrix organiza5on, immune response, defense against fungus

adhesion etc.

IL7- nega5ve regula5on of apopto5c process, posi5ve regula5on of T cell differen5a5on, cell-cell signaling, organ
morphogenesis, bone resorp5on

regula5on of cell prolifera5on

intermediate filament organiza5on

•  EPO- aging, blood circula5on, hemoglobin biosynthe5c process, erythrocyte matura5on, cellular response to hypoxia, posi5ve
•  VIM- astrocyte development, lens fiber cell development, muscle filament sliding, Bergmann glial cell differen5a5on,
•  CLTCL1- anatomical structure morphogenesis, mito5c nuclear division
•  CD9- platelet ac5va5on, brain development, blood coagula5on, cell adhesion
•  ECM1- ossifica5on, angiogenesis, inflammatory response, posi5ve regula5on of angiogenesis, regula5on of T cell migra5on
•  PRNP- learning or memory, axon guidance, cell cycle arrest, nega5ve regula5on of apopto5c process

 

iEXOSOMES are the highly purifies version